A new generation of cross-linkers for selective detection by MALDI MS

作者：Paramelle David; Cantel Sonia; Enjalbal Christine; Amblard Muriel; Forest Eric; Heymann Michael; Geourjon Christophe; Martinez Jean; Subra Gilles^*

来源：Proteomics, 2009, 9(23): 5384-5388.

DOI：10.1002/pmic.200900562

• Cross-linker
• Matrix-assisted laser desorption
• Protein structure prediction
• Signal enhancement
• Technology
• UV-absorbing label

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged crosslinker JMV 3378 in conjunction with a neutral MALDI matrix alpha-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the alpha-cyano-4-hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome G and apo-myoglobine as model proteins.

• 出版日期2009-12
• 单位中国地震局