Anti-hyperlipidemic activity of Rhynchosia nulubilis seeds pickled with brovvn rice vinegar in mice fed a high-fat diet

作者：Park Ki Moon; Lee Seung Ho^*

来源：Nutrition Research and Practice, 2013, 7(6): 453-459.

DOI：10.4162/nrp.2013.7.6.453

• Hyperlipidemia
• Rlrynchosia nulubilis seeds
• brown rice vinegar
• high-fat diet
• epididymis cell

The abnormal content of blood lipids often results in metabolic diseases, such as hyperlipidemia and obesity. Many agents, including natural sources from traditional food, have been developed to regulate the blood lipid contents. In this study, we examined the anti-hyperlipidemic activity of Rhynchosia nulubilis seeds pickled with brown rice vinegar (RNSpBRV), a Korean traditional pickled soybean food. Since RNSpBRV is made of R. nulubilis seeds (ENS) soaked in brown rice vinegar (BRV), we compared the anti-adipogenic activity between ENS, BRV and solid fraction of RINSpBRV (SF-RNSpBRV), liquid fraction of RNSpBRV (LF-RNSpBRV). For this, the inhibitory effect of lipid accumulation in 3T3-L1 adipocyte was checked by adding methanol extracts of mixed ENS and BRV, LF-RNSpBRV, and SF-RNSpBRV. The addition of each methanol extract up to 1 mg/m1 showed no cytotoxicity on 3T3-L1 aclipocyte, and approximately 20% of the lipid droplet formation was suppressed with the methanol extract of BRL or SF-RNSpBRV. The highest suppression (42.1%) was achieved with LF-RNSpBRV. In addition, mice fed a high fat diet (HFD) supplement,d with 5% RNSpBRV powder led to increased high density lipoprotein (HDL) cholesterol and lower blood glucose, triglyceride, and total cholesterol compared to mice fed with a RFD diet only. Interestingly, the size of the epididymis cells gradually decreased in HFD +1% RNSpBRVand BED + 5% RNSpBRV-fed mice if compared those of HFD-fed mice. Taken together, these results provide evidence that RNSpBRV has a regulatory role in lipid metabolism that is related to hyperlipidemia.

• 出版日期2013-12