科研之友
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摘要
Amyloid beta (A beta) peptides, produced through endo-proteolytic cleavage of amyloid precursor protein, are thought to be involved in the death of neural cells in Alzheimer's disease (AD). Although the mechanisms are not fully known, it has been suggested that disruption of cellular activity due to A beta interactions with the cell membrane may be one of the underlying causes. Here in, we have investigated the interaction between A beta-42 and biomimetic lipid membranes and the resulting perturbations in the lipid vesicles. We have shown that A beta oligomeric species localized closer to the membrane surface. Localization of the fibrillar species of A beta-42, although varied, was not as closely associated with the membrane surface. We have demonstrated that the presence of A beta-42 leads to an increase in membrane surface area, inducing lipid temporal vesicular transformation. Furthermore, we have unequivocally shown that A beta-peptides mediate membrane fusion. Although membrane fusion induced by A beta has been hypothesized/proposed, this is the first time it has been visually captured. This fusion may be one of the mechanisms behind the membrane increase in surface area and the resulting vesicular transformation. We have shown that the longer 'amyloidogenic' isoform causes vesicular transformation more readily, and has a higher membrane fusogenic potential than A beta-40. Although not core to this study, it is hugely interesting to observe the high agreement between membrane dynamics and the reported amyloidogenicity of the peptides and aggregation species opening up the potential role of vesicular dynamics for profiling and biosensing of A beta-induced neuro-toxicity.
- 出版日期2013-4
