Astilbin protects diabetic rat heart against ischemia-reperfusion injury via blockade of HMGB1-dependent NF-kappa B signaling pathway

作者：Diao, Huiling; Kang, ZeChun; Han, Fang^*; Jiang, WangLin

来源：Food and Chemical Toxicology, 2014, 63: 104-110.

DOI：10.1016/j.fct.2013.10.045

• Astilbin
• Myocardial ischemia and reperfusion
• High mobility group box-1 protein
• Nuclear factor kappaB
• Diabetes mellitus

Astilbin, a flavonoid compound was isolated from the rhizome of Smilax china L In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effect of Astilbin on diabetic rats in vivo and elucidated the potential mechanism in vitro. The results showed that Astilbin significantly attenuated hypoxia-induced cell injury in a concentration-dependent manner. Treatment of H9c2 cells with Astilbin at 15 mu M blocked nuclear factor kappaB (NF-kappa B) phosphorylation by blocking High-mobility group box protein 1 (HMGB1) expression. Treatment of diabetic rats with Astilbin by intravenous injection (i.v.) at a single dose of 50 mg/kg protected the rats from myocardial IIR injury as indicated by decreasing infarct volume, improving hemodynamics and reducing myocardial damage, and also lowered serum levels of pro-inflammatory factors, reduced HMGB1 and phosphorylated NF-kappa B expression in ischemic myocardial tissue from diabetic rats. Additionally, treatment of diabetic rats with Astilbin at dose of 50 mg/1<g by i.v. for continuous 14 days attenuated cardiac remodeling in the model myocardial I/R injury. These protective effects suggested that Astilbin might be due to block of the myocardial inflammatory cascade via the HMGB1-dependent NF-kappa B signaling pathway.

• 出版日期2014-1
• 单位滨州医学院