A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination

作者：Imayoshi Itaru^*; Hirano Kyoko; Sakamoto Masayuki; Miyoshi Goichi; Imura Tetsuya; Kitano Satsuki; Miyachi Hitoshi; Kageyama Ryoichiro

来源：Neuroscience Research, 2012, 73(1): 85-91.

DOI：10.1016/j.neures.2012.02.003

• Cre
• Flp
• mTFP1
• Knock-in mice
• Rosa26

Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.

• 出版日期2012-5