An automated analysis pipeline for a large set of ChIP-seq data: AutoChIP

作者：Kim Taemook; Lee Wooseok; Han Kyudong^*; Kang Keunsoo

来源：Genes & Genomics, 2015, 37(3): 305-311.

DOI：10.1007/s13258-014-0260-3

• AutoChIP
• ChIP-seq
• STAT5
• GATA3
• Next-generation sequencing
• NGS

Although there are many applications available for the analysis of chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq), users need some knowledge about the installation, alignment, and peak calling procedures prior to the analysis. Here, we present an easy-to-use application for ChIP-seq analysis called AutoChIP. With AutoChIP, installation of necessary programs, alignment of unmapped reads to a reference genome, and identification of genome-wide binding sites can be done in a single step with a large set of ChIP-seq data. Evaluation of the cocktail algorithm implemented in AutoChIP showed that it outperformed a single ChIP-seq tool in terms of the ratio of motif occurrences and the average height of normalized read density over the identified peaks. In addition, annotation of the identified peaks with the known gene and repeat elements information provides a comprehensive picture of the genome-wide binding sites of given proteins. Overall, AutoChIP provides a comprehensive platform to analyze a large set of ChIP-seq data in one step.

• 出版日期2015-3