Depolymerization of heparin by dielectric barrier discharge: Effect of operating modes and anticoagulant potential analysis of low-molecular-weight products

作者：Yang, Jingfeng^*; Liu, Wenzhuan; Yi, Meng; Song, Shuang; Ai, Chunqing; Zhao, Jun

来源：Chemical Physics Letters, 2018, 708: 194-200.

DOI：10.1016/j.cplett.2018.08.019

• Heparin
• Degradation
• Plasma
• Anticoagulant
• Polysaccharide

Low-molecular-weight heparin and ultra-low-molecular-weight heparin are widely used as anticoagulant and antithrombotic drugs. An atmospheric pressure dielectric-barrier discharge procedure was developed to depolymerize heparin to low-or ultra-low-molecular-weight fragments. Dielectric-barrier discharge treatment of heparin resulted in several well-separated and uniform fragments. The structural character of digested fragments below 3,000 Da was elucidated. Expected digestion products such as sulfated disaccharide, sulfated tetrasaccharide, and pentasaccharides with alternatingly-linked N-acetyl glucosamine and iduronic acid were detected. The anticoagulant potential of the digested heparin was tested. The results indicated that dielectric-barrier discharge treatment can produce various molecular weight (MW) heparin fragments and maintain anticoagulant potency.

• 出版日期2018-9-16
• 单位大连工业大学