Alpha-Phellandrene-Induced DNA Damage and Affect DNA Repair Protein Expression in WEHI-3 Murine Leukemia Cells In Vitro

作者：Lin Jen Jyh; Wu Chih Chung; Hsu Shu Chun; Weng Shu Wen; Ma Yi Shih; Huang Yi Ping; Lin Jaung Geng; Chung Jing Gung^*

来源：Environmental Toxicology, 2015, 30(11): 1322-1330.

DOI：10.1002/tox.22003

• alpha-phellandrene
• WEHI-3 leukemia cells
• DNA damage and repair

Although there are few reports regarding alpha-phellandrene (alpha-PA), a natural compound from Schinus molle L. essential oil, there is no report to show that alpha-PA induced DNA damage and affected DNA repair associated protein expression. Herein, we investigated the effects of alpha-PA on DNA damage and repair associated protein expression in murine leukemia cells. Flow cytometric assay was used to measure the effects of alpha-PA on total cell viability and the results indicated that alpha-PA induced cell death. Comet assay and 4,6-diamidino-2-phenylindole dihydrochloride staining were used for measuring DNA damage and condensation, respectively, and the results indicated that a-PA induced DNA damage and condensation in a concentration-dependent manner. DNA gel electrophoresis was used to examine the DNA damage and the results showed that alpha-PA induced DNA damage in WEHI-3 cells. Western blotting assay was used to measure the changes of DNA damage and repair associated protein expression and the results indicated that alpha-PA increased p-p53, p-H2A. X, 14-3-3-r, and MDC1 protein expression but inhibited the protein of p53, MGMT, DNA-PK, and BRCA-1.

• 出版日期2015-11
• 单位中国医科大学