A model of the isolated perfused rat small intestine

作者：Lautenschlaeger Ingmar; Dombrowsky Heike; Frerichs Inez; Kuchenbecker Solveig Carolin; Bade Steffen; Schultz Holger; Zabel Peter; Scholz Jens; Weiler Norbert^*; Uhlig Stefan

来源：American Journal of Physiology - Gastrointestinal and Liver Physiology, 2010, 298(2): G304-G313.

DOI：10.1152/ajpgi.00313.2009

• intestine physiology
• fluid balance
• platelet-activating factor

Lautenschlager I, Dombrowsky H, Frerichs I, Kuchenbecker S, Bade S, Schultz H, Zabel P, Scholz J, Weiler N, Uhlig S. A model of the isolated perfused rat small intestine. Am J Physiol Gastrointest Liver Physiol 298: G304-G313, 2010. First published November 12, 2009; doi:10.1152/ajpgi.00313.2009.-Intestinal edema remains a serious clinical problem, and novel approaches to study its pathophysiology are needed. It was our aim to develop a long-term stable isolated perfused rat small bowel preparation permitting analysis of vascular, luminal, interstitial, and lymphatic compartments and to demonstrate the utility of this model by studying the effects of the proinflammatory mediator platelet-activating factor (PAF). A temperature-controlled chamber with an integrated balance was designed to perfuse isolated intestines through the mesenteric artery and the gut lumen. Steroids or oxygen carriers were not needed. Functional and morphological integrity of the tissue was preserved for several hours as confirmed by oxygen consumption, venous lactate-to-pyruvate ratio, arterial and venous pH, lactose digestion and galactose uptake, intravascular and luminal pressures, maintained fluid homeostasis, gut motility, and quantitative light microscopic analysis. Administration of PAF caused typical effects such as vasoconstriction, gut atony, and loss of galactose uptake. PAF also elicited a transient loss of 20% of the perfusate liquid from the mesenteric vascular bed, two-thirds of which were transferred to the lumen. All these responses were entirely reversible. This new model provides detailed insights into the physiology of the small intestine and will allow to study fundamental processes such as fluid homeostasis, barrier functions, transport mechanisms, and immune responses in this organ. Using this model, here we show a dramatic and yet reversible response of the rat small bowel to PAF, suggesting luminal water clearance as a novel safety factor in the intestine that may be of clinical relevance.

• 出版日期2010-2