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摘要
Background: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-alpha) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). Results: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 +/- 0.01 to 26.19 +/- 0.64 mu g/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S) 5 alpha-cholestane-3 beta, 4 beta, 6 alpha, 7 alpha, 8 beta, 15 alpha, 16 beta, 26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 +/- 0.08 and 1.35 +/- 0.03 mu M), and for (25S) 5 alpha-cholestane-3 beta, 6 alpha, 8 beta, 15 alpha, 16 beta, 26-hexol (1) and (25S) 5 alpha-cholestane-3 beta, 6 alpha, 7 alpha, 8 beta, 15 alpha, 16 beta, 26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 +/- 0.00 and 1.02 +/- 0.01 mu M). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. Conclusion: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.
- 出版日期2015-2-20
- 单位河北医科大学
