A novel rare sugar inhibitor of murine herpes simplex keratitis

作者：Muniruzzaman Syed; McIntosh Megan; Hossain Ahamed; Izumori Ken; Bhattacharjee Partha S

来源：Journal of Pharmacological Sciences, 2016, 131(2): 126-130.

DOI：10.1016/j.jphs.2016.05.004

• Rare sugar
• Herpes simplex keratitis
• Eye drop
• L-psicose
• HSV-1

Purpose: To determine the therapeutic efficacy of a novel rare sugar, L-psicose, for the treatment of HSV-1 induced herpetic stromal keratitis (HSK) in a mouse eye model.
Methods: One rare sugar L-psicose was assayed for HSV-1 inhibition of in vitro virus adsorption. The IC50 and IC90 values of L-psicose were determined using plaque reduction assay (PRA) in CV-1 cell. Female Balb/c mice were corneally infected with HSV-1, strain KOS-GFP; A topical eye drop treatment of L-psicose was started 24 h after infection and continued four times daily for ten consecutive days. The severity of HSK was monitored by slit lamp examination in a masked fashion and Infectious HSV-1 shedding was determined by PRA.
Results: L-psicose was found to have anti-viral activity in vitro at an IC50 dose of 99.5 mM and an IC90 dose of 160 mM. Topical eye drop treatment with 200 mM L-psicose in PBS solution significantly reduced the severity of HSK compared to the mock treatment group. The in vivo mouse ocular model results of L-psicose therapy correlated with accelerated clearance of virus from eye swabs.
Conclusion: The results suggest that topical treatment with rare sugar L-psicose has efficacy against HSK through inhibition of HSV-1. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

• 出版日期2016-6