Modification with DEAE-dextran, an alternative way to prepare anion-exchange monolithic column with lower pressure drop

作者：Wang Manyi; Xu Jun; Zhou Xin; Tan Tianwei^*

来源：Biochemical Engineering Journal, 2007, 34(1): 76-81.

DOI：10.1016/j.bej.2006.11.014

• monolithic column
• DEAE-dextran
• protein
• separation

Poly(methacrylate-co-ethylene dimethacrylate) monolithic column (100 mm x 4.6 mm i.d.) subsequently modified with DEAE-dextran had been prepared for the separation of proteins with anion-exchange mode. The column, which possessed the specific surface area of 5.3 m(2)/g and immobilized amine group of 9.93 mu mol/g media, showed even better permeability of 607 psi at flow rate of 1445 cm/h in comparison to diethylamine modification procedure. The. dynamic binding capacity of bovine serum albumin (BSA) was 23.8 mg/g media. Fast separation of lysozyme, hemoglobin and BSA on the column was achieved within 3 min at flow rate of 1445 cm/h.

• 出版日期2007-4
• 单位北京化工大学