Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge

作者：Yu, Xia; Jia, Renyong^*; Huang, Juan; Shu, Bin; Zhu, Dekang; Liu, Qing; Gao, Xinghong; Lin, Meng; Yin, Zhongqiong; Wang, Mingshu; Chen, Shun; Wang, Yin; Chen, Xiaoyue; Cheng, Anchun

来源：Veterinary Research, 2012, 43: 56.

DOI：10.1186/1297-9716-43-56

• HEAT-LABILE TOXIN
• B-SUBUNIT
• MICE
• IMMUNIZATION
• EXPRESSION
• ADJUVANT
• GENE
• IMMUNOGENICITY
• INDUCTION
• INFECTION

Orally delivered DNA vaccines against duck enteritis virus (DEV) were developed using live attenuated Salmonella typhimurium (SL7207) as a carrier and Escherichia coli heat labile enterotoxin B subunit (LTB) as a mucosal adjuvant. DNA vaccine plasmids pVAX-UL24 and pVAX-LTB-UL24 were constructed and transformed into attenuated Salmonella typhimurium SL7207 resulting SL7207 (pVAX-UL24) and SL7207 (pVAX-LTB-UL24) respectively. After ducklings were orally inoculated with SL7207 (pVAX-UL24) or SL7207 (pVAX-LTB-UL24), the anti-DEV mucosal and systemic immune responses were recorded. To identify the optimum dose that confers maximum protection, we used different doses of the candidate vaccine SL7207 (pVAX-LTB-UL24) during oral immunization. The strongest mucosal and systemic immune responses developed in the SL7207 (pVAX-LTB-UL24) (10(11) CFU) immunized group. Accordingly, oral immunization of ducklings with SL7207 (pVAX-LTB-UL24) showed superior efficacy of protection (60-80%) against a lethal DEV challenge (1000 LD50), compared with the limited survival rate (40%) of ducklings immunized with SL7207 (pVAX-UL24). Our study suggests that the SL7207 (pVAX-LTB-UL24) can be a candidate DEV vaccine.

• 出版日期2012-7-6
• 单位四川农业大学