Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis

作者：Tjellstroem Henrik; Strawsine Merissa; Silva Jillian; Cahoon Edgar B; Ohlrogge John B

来源：FEBS LETTERS, 2013, 587(7): 936-942.

DOI：10.1016/j.febslet.2013.02.021

• AAE15/16
• Acyl chain activating enzyme
• Cuphea
• Medium chain fatty acid
• Thioesterase
• Triacylglycerol

Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e. g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8: 0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.

• 出版日期2013-4-2