Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase

作者：Taki Masumi^*; Kuroiwa Hiroyuki

来源：Amino Acids, 2015, 47(6): 1279-1282.

DOI：10.1007/s00726-015-1989-y

• L/F-transferase
• Non-natural amino acid (unnatural amino acid)
• Positron emission tomography (PET) probe introduction
• O-(2-fluoromethyl)-L-tyrosine (FMT)
• O-(2-fluoroethyl)-L-tyrosine (FET)
• Enzyme kinetics

This article demonstrates the fastest enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins. It is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-l-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine.

• 出版日期2015-6