Application of open tubular capillary columns coated with zirconium phosphonate for enrichment of phosphopeptides

作者：Xue Yanfeng; Wei Junying; Han Huanhuan; Zhao Liyan; Cao Dong; Wang Jinglan; Yang Xiaoming; Zhang Yangjun^*; Qian Xiaohong

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009, 877(8-9): 757-764.

DOI：10.1016/j.jchromb.2009.02.020

• Open tubular capillary columns
• Zirconium phosphonate
• Phosphopeptides
• Mass spectrometry

A new approach utilizing open tubularcapillary Columns coated with Zirconium phosphonate (ZrP-OTCC) for enrichment of phosphopeptides is described. The experimental conditions: interior diameter, length of capillary and How rate was optimized using tryptic digest of alpha-casein (a phosphoprotein) as a model sample. The ZrP-OTCC was demonstrated to tolerate Urea, sodium dodecyl sulphate (SDS). and NaCl. Further experimental results show that the ZrP-OTCC can trap the phosphopeptides even at the concentration of alpha-casein as low as 10(-8) M. This column has also been successfully coupled online with nano-liquid chromatography for enrichment and then separation of phosphopeptides from a complex sample, and finally analyzed the phosphopeptides by mass spectrometry (MS).

• 出版日期2009-3-15
• 单位天津大学; 北京中医药大学; 北京理工大学