High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

作者：Gabriel Emma M; Douarre Pierre E; Fitzgibbon Siobhan; Clair James; Lucey Brigid; Coffey Aidan; O' Mahony Jim M

来源：Journal of Microbiological Methods, 2012, 90(2): 134-136.

DOI：10.1016/j.mimet.2012.04.002

• High resolution melting
• Staphylococcus epidermidis
• Linezolid resistance
• G2576T mutation

We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 235 rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay.

• 出版日期2012-8