Molecular interactions of natural and synthetic steroids in female hamsters%26apos; flank organs

作者：Cabeza Marisa^*; Naranjo Barak; Heuze Yvonne; Sanchez Araceli; Hernandez Mercedes; Sainz Teresita; Bratoeff Eugene

来源：Journal of Dermatological Science, 2012, 66(2): 119-126.

DOI：10.1016/j.jdermsci.2012.02.014

• Enzymes involved in lipid metabolism
• mRNA expression
• Flank organs
• Levonorgestrel
• Progesterone
• Progesterone derivatives

Background: The initial step of steroidal action on target cells is gene activation; therefore, the quantification of mRNA is a direct method for comparing the role of different steroids in the skin. %26lt;br%26gt;Objective: This study demonstrated the role of several steroids on the mRNA expression encoding for different enzymes involved in the lipid metabolism in hamsters%26apos; flank organs, which are a pilosebaceous complex. %26lt;br%26gt;Methods: To determine the effect of treatments with testosterone (T) progesterone (P), levonorgestrel (LNG), 17 alpha-p-chlorobenzoyloxy-6-chloropregn-4,6-diene-3,20-dione (5) and 17 alpha-p-chlorobenzoyloxy-4,6-pregnadiene-3,20-dione (6); T and/or LNG; T and 5 or 6; P and/or 5 or 6 on the expression of mRNA encoding for lipid enzymes, the steroids were applied to the glands; later, the mRNAs expression for the enzymes was determined by PCR. The binding of 5 and 6 to the progesterone receptor (PR) was also evaluated. %26lt;br%26gt;Results: Treatments with T, LNG, T + LNG, P, T + P, 5, T + 5, T + 6, P, P + 5 and P + 6 increased the mRNA expression for glycerol 3-phosphate acyl transferase (GPAT), beta-hydroxy-beta-methylglutaryl-CoA synthase (HMG-CoA-S), beta-hydroxy-beta-methylglutaryl-CoA reductase (HMG-CoA-R), phosphatidylinositol synthase as compared to the controls. However, squalene synthase was increased with all treatments except with T + 5 and 6; 6 did not significantly increase the expression for GPAT or HMG-CoA-S, however it increased the concentration of HMG-CoA-R enzyme. %26lt;br%26gt;5 and 6 bind to the PR, thus indicating that the effect of these steroids on the mRNA expression could be the result of their binding. %26lt;br%26gt;Conclusion: The lipid metabolism is regulated by several steroids thought different mechanism of action, in flank organs.

• 出版日期2012-5