科研之友
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摘要
Analysis of the literature reveals that metabolic syndrome is invariably linked to microvascular disturbances, such as abnormalities in arteriolar reactivity, capillary recruitment, permeability, and hemorheology. The aim of this study was to assess skin microcirculation under baseline conditions and maximum skin hyperemia in response to heating (vasodilatory capacity) in control rats and in the rat model of metabolic syndrome. Twenty four young female rats were randomly assigned into control group (CG) fed on standard rat show & fructose induced insulin resistance group (FG) fed on fructose enriched show (60% of caloric intake) for 2 months. The skin microcirculation was assessed in the hairless ear of rat by Laser Doppler Flowmetry to measure skin blood flow, frequency of vasomotion waves, (frequency 1: 1-3 cycles/min (endothelial activity), frequency 2: 3-5 cycles/min (sympathetic activity), frequency 3: 5-20 cycles/min (vascular myogenic Activity)) & the Power of vasomotion (in perfusion units PU) in relation to the recorded frequencies. All the parameters were measured at 30 degrees C and after local heating of the skin to 44 degrees C. The results demonstrated a significant increase in body mass index, serum glucose & insulin levels (P<0.05), systolic blood pressure, total cholesterol, low density lipoprotein cholesterol & triglycerides (P<0.05) in addition to a significant increase in nitric oxide, high sensitivity C reactive protein & tumor necrosis factor alpha (P<0.05), in FG compared to CG. So it can be claimed that use of fructose in diet for at least 2 months could be a model for experimentally studying the pathophysiological changes in the metabolic syndrome. Regarding parameters of microcirculation, there was a significant decrease in the % change in blood flow between blood flow at 30 degrees C and that after local heating of the skin to 44 degrees C (P< 0.05) in FG compared to CG indicating impaired maximum skin hyperaemia induced by heating of the skin (vasodilatory capacity). Also FG showed a significant lower frequency values in the mid-range of frequency (frequency-2 i.e sympathetic dependent) at 30 degrees C (P<0.05) and in the mid and high range frequencies (frequency-2 & frequency-3 i.e sympathetic and myogenic dependent) at 44 degrees C (P<0.05) in addition to a significant decrease in the power of vasomotion (PU) at all frequency ranges (power-1, 2, and 3) after local heating of the skin to 44 degrees C in comparison to the CG (P< 0.05). The microvascular dysfunction is a hallmark in our results that may be a potential factor explaining the clustering of several components of the metabolic syndrome & associated cardiovascular complications. Our results strongly suggest that targeting micro vascular and endothelial dysfunctions in patients with metabolic syndrome might help to prevent cardiovascular morbidity in those patients. [Mona Aziz, Ali ElAshmaoui, Nahed S. Mohamed, Manal M. Mahmoud and Mona M. Mohamed Assessment of skin microcirculation and inflammatory markers of metabolic syndrome in a rate model. Life Science Journal, 2011; 8(4): 314 -321] (ISSN: 1097-8135). http://www.lifesciencesite.com.
- 出版日期2011
