Dimethyl sulfoxide induces oxidative stress in the yeast Saccharomyces cerevisiae

作者：Sadowska Bartosz Izabela^*; Paczka Aleksandra; Molon Mateusz; Bartosz Grzegorz

来源：FEMS Yeast Research, 2013, 13(8): 820-830.

DOI：10.1111/1567-1364.12091

• DMSO
• glutathione
• reactive oxygen species
• succinate dehydrogenase

Dimethyl sulfoxide (DMSO) is used as a cryoprotectant for the preservation of cells, including yeast, and as a solvent for chemical compounds. We report that DMSO induces oxidative stress in the yeast. Saccharomyces cerevisiae wt strain EG-103 and its mutants sod1, sod2, and sod1 sod2 were used. Yeast were subjected to the action of 1-14% DMSO for 1h at 28 degrees C. DMSO induced a concentration-dependent inhibition of yeast growth, the effect being more pronounced for mutants devoid of SOD (especially sod1 sod2). Cell viability was compromised. DMSO-concentration-dependent activity loss of succinate dehydrogenase, a FeS enzyme sensitive to oxidative stress, was observed. DMSO enhanced formation of reactive oxygen species, estimated with dihydroethidine in a concentration-dependent manner, the effect being again more pronounced in mutants devoid of superoxide dismutases. The content of cellular glutathione was increased with increasing DMSO concentrations, which may represent a compensatory response. Membrane fluidity, estimated by fluorescence polarization of DPH, was decreased by DMSO. These results demonstrate that DMSO, although generally considered to be antioxidant, induces oxidative stress in yeast cells.

• 出版日期2013-12