Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells?

作者：Capp Edison; Jauckus Julia; Corleta Helena von Eye; Toth Bettina; Strowitzki Thomas; Germeyer Ariane^*

来源：European Journal of Obstetrics & Gynecology and Reproductive Biology, 2011, 158(2): 248-253.

DOI：10.1016/j.ejogrb.2011.05.012

• Akt phosphorylation
• Endometrium
• Insulin receptor
• IGF receptor
• Metformin

Objective: To assess the effects of metformin on insulin-, IGF I-, and IGF II-receptor gene expression and Akt phosphorylation in decidualized human endometrial stromal cells (ESC) after stimulation with insulin, IGF I and II.
Study design: ESC were isolated from healthy, regularly cycling women and after two passages decidualized with estrogen/progesterone +/- metformin. Cells were incubated with insulin, IGF I or IGF II for 1, 5, and 10 min to assess Akt phosphorylation by Western blot. To investigate the insulin-, IGF l- and IGF II-receptor gene expression ESC were incubated with insulin, IGF I or IGF II for 6 and 24 h.
Results: Insulin- and IGF I-receptor gene expression in ESC changed significantly after incubation with insulin, IGF I or IGF II. This was further augmented in metformin pretreated cells, while IGF II-receptor gene expression changed particularly after pretreatment with metformin. Akt phosphorylation peaked after 5 min insulin, IGF I and IGF II stimulation in ESC in both control (control 0.08 +/- 0.03 vs. insulin 0.74 +/- 0.19, IGF I 0.68 +/- 0.22, IGF II 0.53 +/- 0.13, p < 0.05) and metformin pretreated cells (control 0.03 +/- 0.01 vs. insulin 0.75 +/- 0.11, IGF I 0.74 +/- 0.15, IGF II 0.67 +/- 0.09. p < 0.005). However, there was no significant difference between the control and metformin pretreated group.
Conclusion: Insulin, IGF I and IGF II lead to changes in their receptor gene expression and induced Akt phosphorylation in ESC. These effects were further highlighted in the presence of metformin.

• 出版日期2011-10