A chip-type thin-layer electrochemical cell coupled with capillary electrophoresis for online separation of electrode reaction products

作者：He, Jian Bo^*; Cui, Ting; Zhang, Wen Wen; DengAnhui, Ning

来源：Analytica Chimica Acta, 2013, 786: 159-165.

DOI：10.1016/j.aca.2013.05.035

• Capillary electrophoresis
• Thin-layer electrolytic cell
• Electrolysis product
• Online separation
• Quercetin

A coupling technique of thin-layer electrolysis with high-performance capillary electrophoresis/UV-vis technique(EC/HPCE/UV-vis) is developed for online separation and determination of electrode reaction products. A chip-type thin-layer electrolytic (CTE) cell was designed and fabricated, which contains a capillary channel and a background electrolyte reservoir, allowing rapid electrolysis, direct sampling and online electrophoretic separation. This chip-type setup was characterized based on an electrophoresis expression of Nernst equation that was applied to the redox equilibrium of o-tolidine at different potentials. The utility of the method was demonstrated by separating and determining the electro-oxidation products of quercetin in different pH media. Two main products were always found in the studied time, potential and pH ranges. The variety of products increased not only with increasing potential but also with increasing pH value, and in total, at least 13 products were observed in the electropherograms. This work illustrates a novel example of capillary electrophoresis used online with thin-layer electrolysis to separate and detect electrode reaction products.

• 出版日期2013-7-5
• 单位合肥工业大学