A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens alpha-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis

作者：Alimolaei Mojtaba; Golchin Mehdi; Ahshenas Jalil; Ezatkhah Majid; Bafti Mehrdad Shamsaddini

来源：Probiotics and Antimicrobial Proteins, 2018, 10(2): 251-257.

DOI：10.1007/s12602-017-9276-8

• Clostridium perfringens
• Lactobacillus casei
• Probiotic
• Vaccine
• Toxoid

The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin (cpa(247-370)) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-alpha strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy. BALB/c mice, immunized orally by the recombinant LC-alpha strain, elicited mucosal and significantly humoral immune responses (p < 0.05) and developed a protection against 900 MLD/mL of the standard alpha-toxin. This study showed that this recombinant LC-alpha strain could be a promising vaccine candidate against gas gangrene and necrotic enteritis.

• 出版日期2018-6