APOBEC2 mRNA and protein is predominantly expressed in skeletal and cardiac muscles of chickens

作者：Li, Juan; Zhao, Xiao-Ling; Gilbert, Elizabeth Ruth; Li, Di-Yan; Liu, Yi-Ping; Wang, Yan; Zhu, Qing^*; Wang, Ya-Gang; Chen, Yang; Tian, Kai

来源：Gene, 2014, 539(2): 263-269.

DOI：10.1016/j.gene.2014.01.003

• Chicken
• APOBEC2
• mRNA/protein expression
• Real-time PCR
• Western blotting

Apolipoprotein B mRNA-editing enzyme catalytic subunit 2 (APOBEC2) plays an important role in regulating and maintaining muscle development in mammals. In this study, we evaluated APOBEC2 mRNA abundance and protein expression and the results indicated that APOBEC2 mRNA was most abundant in skeletal and cardiac muscle, with relatively low expression in the gonads, gizzard and subcutaneous fat tissues of chickens. Immunoreactive APOBEC2 was localized to the cell nucleus of developing myocardium and skeletal myofibers. There were significant differences in mRNA and protein abundance among ages, tissues, and between males and females. In conclusion, APOBEC2 was expressed as the greatest in skeletal muscle and cardiac muscle where it localized to the nucleus. Thus, APOBEC2 may play an important role in muscle development in chickens.

• 出版日期2014-4-15
• 单位四川农业大学