Comparison of automated multiplexed bead-based ANA screening assay with ELISA for detecting five common anti-extractable nuclear antigens and anti-dsDNA in systemic rheumatic diseases

作者：Kim Yoonjung; Park Yongjung; Lee Eun Young; Kim Hyon Suk^*

来源：Clinica Chimica Acta, 2012, 413(1-2): 308-311.

DOI：10.1016/j.cca.2011.10.017

• Multiplex bead-based assay
• BioPlex (TM) 2200 system
• Autoantibodies
• Systemic lupus erythematosus
• Sjogren syndrome

Background: A newly developed and totally automated Luminex-based assay, the BioPlex (TM) 2200 system, is able to detect various autoantibodies simultaneously from a single sample. We compared the BioPlex (TM) 2200 system with ELISA for the detection of six autoantibodies.
Methods: A total of 127 serum samples from the patients with systemic rheumatic diseases were collected and assayed with the BioPlex (TM) 2200 system (Bio-Rad, USA) and conventional ELISA (INOVA Diagnostics, USA) for 5 anti-extractable nuclear antigens. Additionally, relative sensitivity of the BioPlex (TM), 2200 system for detecting anti-dsDNA was evaluated with 79 specimens from SLE patients, which were positive for anti-dsDNA by ELISA.
Results: The concordance rates between ELISA and the BioPlex ranged from 88.1% for anti-RNP to 95.2% for anti-Scl-70, and the kappa coefficients between the results by the two assays were from 0.48 to 0.67. Among the 79 anti-dsDNA positive specimens by ELISA, seventy-eight (98.7%) showed positive results for anti-dsDNA by the BioPlex.
Conclusions: The BioPlex (TM) 2200 system showed comparable results with those by conventional ELISA for detecting autoantibodies, and this automated assay could measure multifarious autoantibodies concurrently in a single sample. It could be effectively used in clinical laboratories for screening autoimmune diseases.

• 出版日期2012-1-18