A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piaui, Brazil

作者：Silvestre Ellida de Aguiar^*; Costa Marcio da Silva; da Silva Pollyana Oliveira; Bajay Miklos Maximiliano; Pinheiro Jose Baldin; Zucchi Maria Imaculada; Guimaraes Campelo Jose Elivalto; Britto Fabio Barros

来源：Brazilian Journal of Animal Science, 2015, 44(4): 155-160.

DOI：10.1590/S1806-92902015000400004

• endogamy
• genetic structure
• microsatellite
• molecular markers

The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piaui (Teresina, Jose de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene differentiation (G(ST),) indicated that 11.9% of the genetic variation was distributed among populations, and according to the coefficient of inbreeding (G(IS) = 0.23 and F-IS = 0.23), there is a deficiency of heterozygotes within populations. These findings corroborate the Bayesian analyses performed with the STRUCTURE software, which revealed three distinct and moderately structured groups. The graphic analysis showed that Teresina and Jose de Freitas are isolated groups, while Angical and Campo Maior share most of their alleles. Despite this, the level of diversity among herds was low. Based on this genetic structure, exchange of reproducers among municipalities is recommended for the maintenance of the breed.

• 出版日期2015-4