Acetyl-keto-beta-boswellic acid induces lipolysis in mature adipocytes

作者：Liu Jian Jun^*; Toy Wan Ching; Liu Sylvia; Cheng Anton; Lim Boon Khim; Subramaniam Tavin; Sum Chee Fang; Lim Su Chi

来源：Biochemical and Biophysical Research Communications, 2013, 431(2): 192-196.

DOI：10.1016/j.bbrc.2012.12.136

• Adipocyte
• Lypolysis
• Acetyl-keto-beta-boswellic acid
• PPAR-gamma

Recently, it was reported that naturally occurring pentacyclic triterpenoids such as ursolic acid have anti-adiposity property. We studied if acetyl-keto-beta-boswellic acid (AKBA), an established anti-inflammation and anti-cancer pentacyclic triterpenoid which has similar chemical structure to ursolic acid, may modulate adipocyte phenotype. 3T3-L1 murine adipocytes and human subcutaneous adipocytes were treated with AKBA in different concentrations in vitro. AKBA triggered significant lipolysis in 3T3-L1 adipocytes as shown by reduced neutral lipids in cytosol and increased free fatty acids in culture medium. Increased lipolysis by AKBA was accompanied by up-regulation of lipolytic enzymes, adipocyte triglyceride lipase (ATGL) and hormone sensitive lipase (HSL),,and a decreased expression of lipid droplet stability regulator perilipin. In addition, AKBA treatment reduced phenotypic markers of mature adipocyte aP2, adiponectin and glut-4 in mature adipocytes. Further studies revealed that AKBA down-regulated PPAR-gamma and C/EBP-alpha expression in a dose and temporal dependent manner in mature adipocytes. In human adipocytes, AKBA likewise mobilized lipolysis accompanied by down-regulation of PPAR-gamma 2 expression and loss of phenotypic markers of mature adipocytes.

• 出版日期2013-2-8