Antitumor activity of Portulaca oleracea L. polysaccharides against cervical carcinoma in vitro and in vivo

作者:Zhao, Rui; Gao, Xu*; Cai, Yaping; Shao, Xingyue; Jia, Guiyan; Huang, Yulan; Qin, Xuegong; Wang, Jingwei; Zheng, Xiaoliang
来源:Carbohydrate Polymers, 2013, 96(2): 376-383.
DOI:10.1016/j.carbpol.2013.04.023

摘要

Portulaca oleracea L has been used as folk medicine in different countries to treat different ailments in humans. P. oleracea L polysaccharide (POL-P), extracted from P. oleracea L, is found to have bioactivities such as hypoglycemic and hypolipidemic activities, antioxidant and antitumor activities. In our study, a water-soluble polysaccharide (POL-P3b) was successfully purified from Galium verum L. by DEAE cellulose and Sephadex G-200 column chromatography. To evaluate the anticancer efficacy and associated mechanisms of POL-P3b on cervical cancer in vitro and in vivo, we showed that treatment of HeLa cell with POL-P3b inhibited cell proliferation. In addition, POL-P3b significantly inhibited tumor growth in U14-bearing mice. Further analysis indicated that POL-P3b possesses the activity of inhibiting cervical cancer cell growth in vitro and in vivo at a concentration- and time-dependent manner, and the mechanisms were associated with Sub-G1 phase cell cycle arrest, triggering DNA damage and inducing apoptosis.

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