ALLIUM TUBEROSUM IMPROVES HEPATIC STEATOSIS VIA INHIBITION OF HISTONE ACETYLATION

作者：Shin Eun Ju; Choi Hyo Kyoung; Hur Haeng Jeon; Park Jae Ho; Sung Mi Jeong; Lee Ae sin; Hur Jin Young; Kim Myung Sunny; Um Soo Jong; Hwang Jin Taek

来源：Current Topics in Nutraceutical Research, 2017, 15(1): 1-8.

• Allium tuberosum
• Steatosis
• Histone acetyl transferase
• HepG2 cell
• High fat diet

Allium tuberosum is widely cultivated in Asian countries and has been reported to be effective for the prevention of certain diseases, including atherosclerosis. The current study was designed to evaluate the inhibitory effects of ethanol extract of Allium tuberosum on histone acetyltransferase activity and non-alcoholic fatty liver disease (hepatic steatosis). The inhibitory effect of a 70% ethanol extract of Allium tuberosum (EAT) on histone acetyltransferase (HAT) activity was evaluated by colorimetric assay and autoradiography. EAT significantly inhibited the activity of histone acetyltransferase in a dose-dependent manner. To examine whether EAT can reduce hepatic steatosis, we used oleic acid (OA)-induced HepG2 cell model of steatosis. EAT 100 and 200 mu g/mL, significantly reduced OA-induced lipid accumulation in HepG2 cells. The expression of PPAR-alpha was also increased by EAT, which did not show significant cytotoxicity until 400 mu g/mL concentrations in HepG2 cells. We further demonstrated that OA treatment on HepG2 cell caused a significant increase in histone H3 lysine 9 (H3K9) acetylation. EAT significantly decreased the OA increased H3K9 acetylation in HepG2 cells. Finally, we also investigated the hypolipidenzic effect of EAT using mice fed a high-fat diet (HFD). Administration of 2% EAT significantly reduced the HFD-induced increase in body weight, liver weight, subcutaneous fat, fatty liver and adipose tissue size. These results suggest that Allium tuberosum may be useful for the prevention of hepatic steatosis and is possibly mediated by the inhibition of histone acetylation.

• 出版日期2017