Docosahexaenoic acid prevents dendritic cell maturation, inhibits antigen-specific Th1/Th17 differentiation and suppresses experimental autoimmune encephalomyelitis

作者：Kong Weimin; Yen Jui Hung; Ganea Doina^*

来源：Brain, Behavior, and Immunity, 2011, 25(5): 872-882.

DOI：10.1016/j.bbi.2010.09.012

• Docosahexaenoic acid
• Experimental autoimmune
• encephalomyelitis
• IL-12
• IL-23
• Th1
• Th17
• Foxp3
• Tbet
• ROR gamma t

Docosahexaenoic acid (DHA), the most abundant essential n - 3 polyunsaturated fatty acid in the CNS, emerged recently together with eicosapentaenoic acid (EPA) and DHA/EPA metabolic derivatives as a major player in the resolution of inflammation. Protective anti-inflammatory effects of DHA were reported in clinical studies and animal models of colitis, sepsis, and stroke. Here we report for the first time a beneficial effect of dietary n - 3 fatty acids in experimental autoimmune encephalomyelitis (EAE), a model for human multiple sclerosis. In the present study we investigated the effects of DHA on the function of bone marrow-derived dendritic cells (DC) in CD4(+) T cell stimulation and differentiation. Pretreatment of DC with DHA prevented LPS-induced DC maturation, maintaining an immature phenotype characterized by low expression of costimulatory molecules and lack of proinflammatory cytokine production (IL-12p70, IL-6, and IL-23). DHA-treated DC were poor stimulators of antigen-specific T cells in terms of proliferation and Th1/Th17 differentiation. This was associated with an increase in p27(kip1), a cell cycle arresting agent, and with decreases in Tbet. GATA-3, and ROR gamma t, master transcription factors for Th1, Th2, and Th17. In contrast, T cells co-cultured with DC-DHA express higher levels of TGF beta and Foxp3, without exhibiting a functional Treg phenotype. Similar to the in vitro results, the beneficial effect of DHA in EAE was associated with reduced numbers of IFN gamma- and IL-17-producing CD4(+) T cells in both spleen and CNS.

• 出版日期2011-7