Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

作者：Reinardy H C; Skippins E; Henry T B^*; Jha A N

来源：Journal of Fish Biology, 2013, 82(3): 1074-1081.

DOI：10.1111/jfb.12042

• comet assay
• DNA strand breaks
• hydrogen peroxide
• repeated sampling
• single-cell gel electrophoresis assay

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.

• 出版日期2013-3