A new disease in Tabernaemontana associated with Tobacco mild green mosaic virus

作者：Cohen J; Rosner A; Kagan S; Lampel M; Maslenin L; Zeidan M; Gera A^*

来源：Annals of Applied Biology, 2001, 138(2): 153-159.

DOI：10.1111/j.1744-7348.2001.tb00097.x

• tobamovirus
• detection
• Apocynaceae
• electron microscopy
• sequence

Conspicuous viral symptoms were seen on Tabernaemontana divaricata, a member of the family Apocynaceae, grown in a commercial nursery, in Israel. The symptoms varied widely and included chlorotic ringspots and banding, oak-leaf patterns and mosaic. At the end of the winter, large yellow spots, which later became necrotic, appeared on fully expanded leaves. The necrotic zones later fell out, leaving "shot holes". Preliminary analysis suggested that the disease was associated with a tobamovirus. Particles typical of a tobamovirus were observed by electron microscopy only in samples taken from symptomatic leaves. A partial segment of the 5'-terminus of the viral RNA, which comprised of 533 by was cloned and sequenced. Comparison of the predicted amino acid sequence with those of other tobamoviruses revealed 94% identity with Tobacco mild green mosaic virus (TMGMV) genome. Primers specific to the coat protein (CP) gene of TMGMV used in a reverse transcription-polymerise chain reaction assay (RT PCR) gave an expected amplification product of 455 bp. The amino acid composition of the cloned CP gene, which shows a complete identity to that of TMGMV confirmed the identity of TMGMV infecting Tabernaemontana. Polyclonal antibodies prepared against the virus and used in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) enabled specific detection of the virus in crude sap extracted from T. divaricata and mechanically inoculated indicator plants. This is the first report of TMGMV infection in Tabernaemontana and the first incidence of the virus in Israel.

• 出版日期2001