Molecular evidence of hybrid zones of Cedrela (Meliaceae) in the Yungas of Northwestern Argentina

作者：Zelener Noga^*; Tosto Daniela; de Oliveira Luiz Orlando; Cristina Soldati Maria; Virginia Inza Maria; Fernando Fornes Luis

来源：Molecular Phylogenetics and Evolution, 2016, 102: 45-55.

DOI：10.1016/j.ympev.2016.05.020

• Amplified Fragment Length Polymorphism (AFLP)
• Cedrela
• Genetic structure
• Hybrid zones
• Internal Transcribed Spacer (ITS)

In the Yungas of Northwestern Argentina, three endangered species of Cedrela (C. angustifolia, C saltensis, and C balansae) follow altitudinal gradients of distribution with contact zones between them. We sampled 210 individuals from 20 populations that spanned most of Cedrela's geographical range in the Yungas, and used Amplified Fragment Length Polymorphism (AFLP) markers and DNA sequences of the nuclear Internal Transcribed Spacer (ITS) to investigate hybrid zones. Data analyses employed an array of complementary methods, including principal coordinate analyses, Bayesian clustering analyses, maximum likelihood tree-building, and network techniques. Both nuclear molecular systems - AFLP and ITS - provided insights into the evolutionary history of Cedrela in the Yungas in a congruent manner. We uncovered strong support for the occurrence of natural hybridization between C balansae and C. saltensis. Additionally, we identified hybrid zones in areas of sympatry (at both the Calilegua National Park and the San Andres farm) and in transition zones from 820 to 1100 meters above sea level (localities of Pintascayo and Acambuco). There was no evidence for hybridization of either C balansae or C saltensis with C angustifolia. The role of hybrid populations in conservation and use of genetic resources in the Yungas were discussed.

• 出版日期2016-9