Automated Genotyping of a Highly Informative Panel of 40 Short Insertion-Deletion Polymorphisms Resolved in Polyacrylamide Gels for Forensic Identification and Kinship Analysis

作者：Pena Heloisa B; Pena Sergio D J^*

来源：Transfusion Medicine and Hemotherapy, 2012, 39(3): 211-216.

DOI：10.1159/000338854

• Insertion-deletion polymorphisms
• Indels
• DNA
• Identification
• Genome
• Polyacrylamide gels

Objective: Short insertion-deletion polymorphisms (indels) are the second most abundant form of genetic variations in humans after SNPs. Since indel alleles differ in size, they can be typed using the same methodological approaches and equipment currently utilized for microsatellite genotyping, which is already operational in forensic laboratories. We have previously shown that a panel of 40 carefully chosen indels has excellent potential for forensic identification, with combined probability of identity (match probability) of 7.09 x 10(-17) for Europeans. Methods: We describe the successful development of a multiplex system for genotyping the 40-indel panel in long thin denaturing polyacrylamide gels with silver staining. We also demonstrate that the system can be easily fully automated with a simple large scanner and commercial software. Results and Conclusion: The great advantage of the new system of typing is its very low cost. The total price for laboratory equipment is less than EUR 10,000.-, and genotyping of an individual patient will cost less than EUR 10.-in supplies. Thus, the 40-indel panel described here and the newly developed %26apos;low-tech%26apos; analysis platform represent useful new tools for forensic identification and kinship analysis in laboratories with limited budgets, especially in developing countries.

• 出版日期2012-6