Association of ectomycorrhizal fungi with Picea crassifolia (Pinaceae, Piceoidae) from high-altitude stands in Mount Helan Nature Reserve, China

作者：Fan, Y. J.; Grebenc, T.; Wei, J.; Zhao, Y. L.; Yan, W.; Wang, L. B.^*

来源：Genetics and Molecular Research, 2016, 15(3): 15038604.

DOI：10.4238/gmr.15038604

• Mount Helan (China)
• Diversity
• Ectomycorrhizal
• Morphological and molecular identification
• ITS nrDNA sequencing
• Picea crassifolia Kom. (Qinghai spruce) stand

We investigated the diversity of ectomycorrhiza associated with the endemic Picea crassifolia in Mount Helan National Nature Reserve in Inner Mongolia, China. Toward this objective, we conducted morphological and molecular identification of ectomycorrhizae in soil cubes taken from pure P. crassifolia stands. Eleven types of ectomycorrhizal (ECM) organisms were separated, briefly described, and identified. Nine morphotypes belonged to the phylum Basidiomycotina [Amphinema byssoides, Cortinarius sp (cf. limonius), Cortinarius vernus, Inocybe cf. nitidiscula, Inocybe sp 1, Sebacina incrustans, Sebacina sp, Suillus luteus, and Piceirhiza tuberculata x Picea crassifolia (comb. Nov.)], and two morphotypes to the phylum Ascomycotina (Cenococcum geophilum and Helvella sp). The diversity of ECM organisms in P. crassifolia was lower than that reported by other studies on spruce or pine forests, or on sporocarp diversity in the high-mountain forests of China. Most of the fungi in the rhizosphere did not correspond to species previously recorded as sporocarps above ground. Here, several new ectomycorrhiza morphotypes are proposed and described. We also confirmed the ectomycorrhizal status of the genus Sebacina (order Sebacinales).

• 出版日期2016
• 单位中国林业科学研究院林业研究所; 内蒙古农业大学; 内蒙古科技大学包头师范学院