Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

作者：Li Na; Zhou Jian Zhang^*; Lin Ling Ling; Han Nan Nan; Lin Zhong Hua

来源：Acta Physico - Chimica Sinica, 2010, 26(9): 2468-2472.

• Au nanoparticle
• Colorimetric detection
• Conformational changes of Cyt c
• pH value
• L-Cys

The colors of Au nanoparticles (Au NPs) change along with conformational changes in cytochrome c (Cyt c). We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H(+) and L-cysteine (L-Cys). We improved the conventional procedure for this detection. After the addition of Cyt c within different pH values, the Au NPs are either cyan, blue, purple or red. This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH. induced conformational changes in Cyt c. At pH 7 the color of Au NPs changes from purple to blue and then cyan upon the addition of L-Cys, which suggests that the Au NPs can be used for the colorimetric detection of Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).

• 出版日期2010-9
• 单位厦门大学