Analysis of mandipropamid residual levels through systematic method optimization against the matrix complexity of sesame leaves using HPLC/UVD

作者：Farha Waziha; Rahman Md Musfiqur; Abd El Aty A M^*; Kim Sung Woo; Jung Da I; Im So Jeong; Choi Jeong Heui; Kabir Md Humayun; Lee Kang Bong; Shin Ho Chul; Shim Jae Han^*

来源：Biomedical Chromatography, 2016, 30(7): 990-995.

DOI：10.1002/bmc.3638

• Mandipropamid
• Sesame leaves
• Method development
• Residue analysis
• Dissipation patter
• Half-life

An analytical method was developed to detect mandipropamid residues in sesame leaves using high-performance liquid chromatography-ultraviolet detection. Samples were extracted with acetonitrile and were prepurified using a solid-phase extraction (SPE) cartridge with an additional dispersive-SPE (d-SPE) sorbent application. The method was validated using an external calibration curve prepared using pure solvent. The linearity was excellent with determination coefficient = 1. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Recoveries at three spiking levels - 0.1, 0.5, and 1.0 mg/kg - were in the range 80.3-90.7% with relative standard deviations <2%. This method was applied to field-treated samples collected from two different areas, Gwangju and Muan, in the Republic of Korea and the half-lives were similar, 5.10 and 5.41 days, respectively. The pre-harvest residue limit was also predicted for both sites. The proposed method is sensitive and able to quantify trace amounts of mandipropamid in leafy vegetables. The combination of SPE and d-SPE effectively removed the matrix components in sesame leaves.

• 出版日期2016-7