Nestin expression and glial response in the hippocampus of mice after trimethyltin treatment

作者：Lee Sueun; Yang Miyoung; Kim Jinwook; Kim Juhwan; Son Yeonghoon; Kwon Seungjoo; Kim Sung Ho; Kim Jong Choon; Kang Seong Soo; Wang Hongbing; Shin Taekyun^*; Moon Changjong

来源：Acta Histochemica, 2014, 116(8): 1276-1288.

DOI：10.1016/j.acthis.2014.07.009

• Nestin
• Hippocampus
• Trimethyltin
• Dentate gyrus
• Astrocytes
• Mice

Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically induced hippocampal injury. TMT treatment (2.5 mg/kg, intraperitoneally) selectively injured the dentate gyrus (DG) of the mouse hippocampus. The level of hippocampal mRNA encoding nestin increased significantly 2 and 3 days post-treatment and thereafter decreased (at 4 and 8 days post-treatment). The level of nestin protein significantly increased 2 - 4 days post-treatment, particularly in the injured region of the DG, and predominantly in glial fibrillary acidic protein-positive astrocytes in the hippocampal DG. Ki67-positive proliferating cells were increased following TMT treatment and colocalized with nestin-positive reactive astrocytes. Thus, we suggest that nestin contributes to remodeling of the chemically injured DG via glial scar formation and the alteration of neurogenesis.

• 出版日期2014