Development of an Immunoaffinity Chromatography Purification and Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Method for Determination of 12 Sulfonamides in Beef and Milk

作者：Zhao Xiao Tian; Lin Qin Bao^*; Song Huan; Pan Ya Li; Wang Xiong

来源：Journal of Agricultural and Food Chemistry, 2011, 59(18): 9800-9805.

DOI：10.1021/jf202705d

• immunoaffinity chromatography (IAC)
• ultra performance liquid chromatography (UPLC)
• mass spectrometry (MS)
• sulfonamide
• beef
• milk

A highly selective and sensitive method was developed for the simultaneous determination of 12 sulfonamides in beef and milk by immunoaffinity chromatography purification coupled to ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The MS/MS conditions, UPLC mobile phase, injection solution, sample purification process, and matrix effect were studied to optimize the operating conditions. The limits of detection (LODs) of the instrument for the studied sulfonamides ranged from 0.4 to 2.0 mu g L(-1), being 1.6-8.0 mu g kg(-1) for beef and 1.8-6.4 mu g kg(-1) for milk. The standard solution was diluted with blank beef or milk matrix for the construction of calibration curves, which had a linear range from 10 to 200 mu g kg(-1) and regression coefficients higher than 0.990 (n = 10) for all the studied sulfonamides. Samples spiked at 10, 20, and 100 mu g kg(-1) showed recoveries above 70% and relative standard deviations below 10%.

• 出版日期2011-9-28
• 单位山西大学; 中国科学院长春应用化学研究所