Accelerated Dual-Contrast First-Pass Perfusion MRI of the Mouse Heart: Development and Application to Diet-Induced Obese Mice

作者：Naresh Nivedita K; Chen Xiao; Roy Rene J; Antkowiak Patrick F; Annex Brian H; Epstein Frederick H^*

来源：Magnetic Resonance in Medicine, 2015, 73(3): 1237-1245.

DOI：10.1002/mrm.25238

• perfusion reserve
• MRI
• high-fat diet
• first-pass MRI
• mice
• cardiac

PurposeGene-modified mice may be used to elucidate molecular mechanisms underlying abnormal myocardial blow flow (MBF). We sought to develop a quantitative myocardial perfusion imaging technique for mice and to test the hypothesis that myocardial perfusion reserve (MPR) is reduced in a mouse model of diet-induced obesity (DIO).
MethodsA dual-contrast saturation-recovery sequence with k(y)-t undersampling and a motion-compensated compressed sensing reconstruction algorithm was developed for first-pass MRI on a small-bore 7 Tesla system. Control mice were imaged at rest and with the vasodilators ATL313 and Regadenoson (n=6 each). In addition, we imaged mice fed a high-fat diet (HFD) for 24 weeks.
ResultsIn control mice, MBF was 5.70.8 mL/g/min at rest and it increased to 11.8 +/- 0.6 mL/g/min with ATL313 and to 10.4 +/- 0.3 mL/g/min with Regadenoson. In HFD mice, we detected normal resting MBF (5.6 +/- 0.4 versus 5.0 +/- 0.3 on control diet), low MBF at stress (7.7 +/- 0.4 versus 10.4 +/- 0.3 on control diet, P<0.05), and reduced MPR (1.4 +/- 0.2 versus 2.0 +/- 0.3 on control diet, P<0.05).
ConclusionAccelerated dual-contrast first-pass MRI with motion-compensated compressed sensing provides spatiotemporal resolution suitable for measuring MBF in free-breathing mice, and detected reduced MPR in DIO mice. These techniques may be used to study molecular mechanisms that underlie abnormal myocardial perfusion. Magn Reson Med 73:1237-1245, 2015.

• 出版日期2015-3