NLRP3 RNAi of Injected Synoviocytes Inhibits the Inflammatory Responses in Articular Cavity and Alleviates the Injury of Articular Cartilage in a Rabbit Model

作者：Zhang, Hua^*; Yang, Junxing^*; Zhou, Bengen; Chen, Jianfa; Li, Jie; Li, Qihuo; Xiong, Hao; Liu, Fuyifei

来源：Journal of Biomaterials and Tissue Engineering, 2018, 8(7): 1010-1016.

DOI：10.1166/jbt.2018.1837

• Post-Traumatic Arthritis
• NLRP3
• Inflammation

The aim of this study was to evaluate the therapeutic effects of NOD-like receptor family, pyrin domain-containing protein 3 (NLRP3) RNAi on a rabbit model of post-traumatic arthritis. New Zealand rabbits underwent bilateral knee surgery to establish a trauma model. Primarily isolated synoviocytes, with or without NLRP3 RNAi or scramble RNAi, were injected into the articular cavity of rabbits. Hematoxylin-eosin staining, safranin-O staining of the articular cartilage, enzymelinked immunosorbent assay, and western blot were used in the research. NLRP3 RNAi significantly inhibited the expressions of both TNF-alpha and IL-1 beta in synoviocytes. The total Mankin score was significantly lowed in NLRP3 RNAi group compared with that in other three groups. Synoviocytes and scramble RNAi group showed significantly increased levels of TNF-alpha, IL-1 beta, IL-6 and IL-8 in synovial fluid compared with control group both at two and four weeks after injection of cells. The level difference of TNF-alpha, 1L-1 beta, IL-6 and IL-8 between NLRP3 RNAi group and other three groups could be statistically notified at only one week after injection of cells, and maintained until four weeks after injection. NLRP3 RNAi of injected synoviocytes effectively inhibits the inflammatory responses in articular cavity and alleviates the injury of articular cartilage.

• 出版日期2018-7
• 单位广州中医药大学; 广州中医药大学第一附属医院