Hydrophilic modification of titania nanomaterials as a biofunctional adsorbent for selective enrichment of phosphopeptides

作者：Liu, Hailong^*; Yang, Tianyi; Dai, Junyong; Zhu, Jiayu; Li, Xiaoran; Wen, Rui; Yang, Xinghao

来源：Analyst, 2015, 140(19): 6652-6659.

DOI：10.1039/c5an01544h

• AFFINITY-CHROMATOGRAPHY PLATFORM
• MASS-SPECTROMETRY
• PHOSPHOPROTEOME ANALYSIS
• MAGNETIC NANOPARTICLES
• MS
• PHOSPHORYLATION
• IMMOBILIZATION
• MICROSPHERES
• GRAPHENE
• MATRIX

TiO2-based metal oxide affinity chromatography (MOAC) nanomaterials show high potential in phosphoproteome mass-spectrometric (MS) analysis. However, a drawback of TiO2 nanomaterials is poor water solubility, which greatly reduces the enrichment efficiency of phosphopeptides and eventually limits their use in phosphoproteome MS analysis. In this work, a hydrophilic TiO2 hybrid material (denoted as NH2@TiO2) is successfully designed with 1,6-hexanediamine modified on the surface of TiO2 nanoparticles and applied as a biofunctional adsorbent for selective enrichment of phosphopeptides. The novel TiO2 hybrid material with high hydrophilicity and biocompatibility is characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and infrared (IR) spectroscopy, and its performance in selective enrichment of phosphopeptides is evaluated with the standard protein digests, human serum and the tryptic digests of nonfat milk.

• 出版日期2015
• 单位南京师范大学; 中国药科大学