Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips

作者：Hataoka Y; Zhang LH^*; Mori Y; Tomita N; Notomi T; Baba Y

来源：Analytical Chemistry, 2004, 76(13): 3689-3693.

DOI：10.1021/ac035032u

We have successfully achieved the integration of isothermal amplification and the subsequent analysis of specific gene fragments on poly(methyl methacrylate) microchips. In our experiments, loop-mediated isothermal amplification, which can offer higher specificity and efficiency than PCR, has been performed at a constant temperature (65 degreesC). After amplification, products could be either examined by the integrated microchip-based electrophoresis or directly observed by naked eye with SYBR Green I added into the reaction solution. By such an integrated microsystem, the amplification and the subsequent analysis of prostate-specific antigen gene with template concentration at 23 fg/muL could be finished within 15 min, which demonstrates its advantages of high specificity, good reproducibility, and fast speed in gene detection.

• 出版日期2004-7-1
• 单位中国科学院; 中国科学院大连化学物理研究所