科研之友
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摘要
The study was carried out to investigate external contamination of hair by blood in heroin-related postmortem cases. Solutions were prepared containing 0.05, 0.1, 0.2, 0.5 and 3.0 mu g/mL of 6-monoacetylmorphine (6-AM) only or morphine only in human blood. Samples of approximately 3.2 g of drug-free hair were contaminated by soaking in the blood solutions for 5 min. They were then removed and left at room temperature. Approximately 0.5 g of hair was collected from each of the blood soaked hair samples at 6 h, 1, 2, 4 and 7 days after contamination. As each hair sample was collected it was shampoo-washed to prevent further drug absorption. Hair samples were analysed in triplicate using a fully validated method described previously. 6-AM broke down to morphine in all samples. In hair contaminated with blood containing 0.05, 0.1 and 0.2 mu g/mL 6-AM or morphine drug was either not detected or was detected below the limit of quantitation (0.2 ng/mg hair) at all contamination times. In hair contaminated with blood spiked with 0.5 mu g/mL morphine, the concentration in hair ranged from 0.54 to 0.91 ng/mg and in hair contaminated with blood spiked with 3.0 mu g/mL, from 3.25 to 5.77 ng/mg. The concentrations of 6-AM ranged from 0.65 to 1.11 ng/mg and morphine from 0.34 to 0.80 ng/mg in hair contaminated with 0.5 mu g/mL 6-AM in blood. 6-AM ranged from 2.12 to 3.67 ng/mg and morphine from 0.84 to 2.05 ng/mg in hair contaminated with 3 mu g/mL 6-AM in blood. For 6-AM and morphine ANOVA statistical evaluation showed no significant difference among the concentrations over time.
- 出版日期2011-7-15
