Bhlhe40 controls cytokine production by Tcells and is essential for pathogenicity in autoimmune neuroinflammation

作者：Lin Chih Chung; Bradstreet Tara R; Schwarzkopf Elizabeth A; Sim Julia; Carrero Javier A; Chou Chun; Cook Lindsey E; Egawa Takeshi; Taneja Reshma; Murphy Theresa L; Russell John H; Edelson Brian T^*

来源：Nature Communications, 2014, 5(1): 3551.

DOI：10.1038/ncomms4551

T(H)1 and T(H)17 cells mediate neuroinflammation in experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis. Pathogenic T-H cells in EAE must produce the pro-inflammatory cytokine granulocyte-macrophage colony stimulating factor (GM-CSF). TH cell pathogenicity in EAE is also regulated by cell-intrinsic production of the immunosuppressive cytokine interleukin 10 (IL-10). Here we demonstrate that mice deficient for the basic helix-loop-helix (bHLH) transcription factor Bhlhe40 (Bhlhe40(-/-)) are resistant to the induction of EAE. Bhlhe40 is required in vivo in a T cell-intrinsic manner, where it positively regulates the production of GM-CSF and negatively regulates the production of IL-10. In vitro, GM-CSF secretion is selectively abrogated in polarized Bhlhe40(-/-) T(H)1 and T(H)17 cells, and these cells show increased production of IL-10. Blockade of IL-10 receptor in Bhlhe40(-/-) mice renders them susceptible to EAE. These findings identify Bhlhe40 as a critical regulator of autoreactive T-cell pathogenicity.

• 出版日期2014-4