DEMONSTRATION OF URR-DUPLICATION VARIANTS OF HUMAN PAPILLOMAVIRUS TYPE-6 IN PARAFFIN-EMBEDDED TISSUE-SECTIONS OF ONE CONDYLOMA ACUMINATUM AND ONE BUSCHKE-LOEWENSTEIN TUMOR

作者：RUBBEN A^*; SPELTEN B; ALBRECHT J; GRUSSENDORFCONEN EI

来源：Journal of Pathology, 1994, 174(1): 7-12.

• HPV 6
• URR
• DNA DUPLICATIONS
• PCR
• PARAFFIN-EMBEDDED TISSUE

Human papillomavirus type 6 (HPV 6) induces condylomata acuminata and laryngeal papillomas. Occasionally, HPV 6 may also be found in low-grade verrucous carcinomas. In some tumours, genetic analysis revealed the presence of HPV 6 variants with rearrangements, mostly DNA duplications, within the upstream regulatory region (URR). In this study, we analysed 98 formalin-fixed, paraffin-embedded condylomata acuminata obtained from 54 patients for the presence of URR-duplication variants of HPV 6. HPV 6 DNA could be amplified by polymerase chain reaction (PCR) from 40 samples. One condyloma acuminatum contained a HPV 6 genome with rearranged URR. Analysis using restriction enzyme cleavage suggested a DNA duplication within the URR of approximately 200 bp, spanning the Hpa II site at nt 7863 and the Dde I site at nt 7843 but not involving the Rsa I site at nt 7633. In addition, we analysed the distribution of the already characterized URR-duplication variant HPV 6(AC1B) within different paraffin-embedded tissue sections of a Buschke-Loewenstein tumour. No correlation could be demonstrated between the presence of the rearranged genome and malignant histological changes. This result and the demonstration of an URR-duplication variant in a typical condyloma acuminatum suggest that duplications within the URR of HPV 6 are not directly related to malignant progression of HPV 6-induced tumours.

• 出版日期1994-9