Nuclear factor kappa B activity correlates with the progression and prognosis of pancreatic cancer in a mouse model

作者：Furukawa Kenei^*; Uwagawa Tadashi; Haruki Koichiro; Fujiwara Yuki; Iida Tomonori; Shiba Hiroaki; Misawa Takeyuki; Ohashi Toya; Yanaga Katsuhiko

来源：Surgery Today, 2013, 43(2): 171-177.

DOI：10.1007/s00595-012-0279-5

• NF-kappa B
• Pancreatic cancer
• Tumor progression
• Prognosis

Constitutive NF-kappa B activation is considered to play a key role in the aggressive behavior of pancreatic cancer. Although NF-kappa B in tumors may contribute to aggressive characteristic features via transcription of angiogenesis and invasion-related factors, there is no definitive evidence showing a correlation between quantitated NF-kappa B activity and prognosis. In this study, we quantitated NF-kappa B activity of various human pancreatic cancer cell lines and evaluated whether NF-kappa B activity was related to tumor progression and prognosis for pancreatic cancer in mice. %26lt;br%26gt;We quantitated NF-kappa B activity in six pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-2, MIAPaCa-2, Panc-1 and PL45) and evaluated downstream target genes of NF-kappa B such as VEGF, IL-8 and MMP-9 in vitro. Next, we evaluated tumor progression and prognosis using subcutaneous tumor model in vivo between cell lines with the highest and lowest NF-kappa B activity. %26lt;br%26gt;BxPC-3 had the highest and AsPC-1 had the lowest NF-kappa B activity in the 6 cell lines. Expression of VEGF, IL-8 and MMP-9 in BxPC-3 was significantly higher than those in AsPC-1 cells in vitro (p %26lt; 0.001) and tumor growth in BxPC-3 was faster than that in AsPC-1 group (p %26lt; 0.001) resulting in worse survival in vivo (p = 0.0339). %26lt;br%26gt;These results suggested that NF-kappa B activity is related to expression of its downstream target genes, tumor progression and prognosis in experimental pancreatic cancer model.

• 出版日期2013-2