A validated H-1 NMR method for quantitative analysis of alpha-bisabolol in essential oils of Eremanthus erythropappus

作者：Cerceau Cristiane I; Barbosa Luiz C A^*; Alvarenga Elson S; Ferreira Antonio G; Thomasi Sergio S

来源：Talanta, 2016, 161: 71-79.

DOI：10.1016/j.talanta.2016.08.032

• qNMR
• COSY
• H-1 NMR
• Gas chromatography
• Essential oils
• alpha-Bisabolol

alpha-Bisabolol is a natural terpene produced by Eremanthus erythropappus and is widely used in cosmetics and pharmaceuticals due to its anti-inflammatory, antibacterial and antimycotic properties. Due to these applications, a control of composition and authenticity of commercial oils rich in this terpene is required, resulting in a demand for new methodologies for quality control. In this work a rapid and efficient method for quantification of alpha-bisabolol in the essential oil of E. erythropappus (candeia) using H-1 NMR was developed, validated and compared to gas chromatography (GC) method. The quantification of alpha-bisabolol by H-1 NMR was successfully achieved for most of the essential oil samples of E. erythropappus evaluated, except for those with a more complex composition. To circumvent this limitation a 2D NMR COSY contour map was used. This method proved to be a fast and efficient alternative, providing results with standard deviations SD <0.3%. All evaluated parameters (selectivity, linearity, accuracy/precision, repeatability, robustness and stability of analyte and internal standard in solution) gave satisfactory results. Using the H-1 NMR signals at 5.36 and 5.13 ppm, the limit of detection (LOD) and limit of quantification (LOQ) were 0.26 and 2.59 mg, respectively. The results obtained by the H-1 NMR method presented SD=0.59%, smaller than the value found for GC (SD=1.18%). Tukey tests have shown that the results obtained by H-1 NMR and COSY methodology are similar to the obtained by the traditional GC-FID technique using external and internal standardization and normalization with 95% confidence.

• 出版日期2016-12-1