Arthrobacter echini sp nov., isolated from the gut of a purple sea urchin, Heliocidaris crassispina

作者：Lee June Young; Hyun Dong Wook; Kim Pil Soo; Kim Hyun Sik; Shin Na Ri; Yun Ji Hyun; Jung Mi Ja; Kim Min Soo; Whon Tae Woong; Bae Jin Woo^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(4): 1887-1893.

DOI：10.1099/ijsem.0.000965

A novel strain, designated AM23(T), was isolated from the gut of a purple sea urchin Heliocidaris crassispina collected from the coastal waters of the Korean island Dokdo. 16S rRNA gene sequence analysis showed that strain AM23(T) belonged to the genus Arthrobacter in the family Micrococcaceae and shared highest sequence similarity with Arthrobacter agilis DSM 20550T (98.77 %). Strain AM23(T) was catalase-positive, oxidase-negative and grew optimally at 20 degrees C, in the presence of 1 % (w/v) NaCl and at pH 7. The isolate was a Gram-stain-positive, non-motile, strictly aerobic and coccus-shaped bacterium. The major cellular fatty acids were anteiso-C-15 : 0 and iso-C-15 : 0. The polar lipids of strain AM23(T) were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and four unidentified lipids. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine and the predominant cell-wall sugars were galactose, mannose, rhamnose and ribose. The major respiratory quinone was identified as menaquinone MK-9(H-2). The genomic DNA G+C content was 67.3 mol% and the DNA-DNA hybridization values showed the strain shared less than 29 % genomic relatedness with A. agilis DSM 20550T. The results of the phylogenetic, phenotypic and genotypic analysis indicate that strain AM23(T) represents a novel species in the genus Arthrobacter, for which the name Arthrobacter echini sp. nov. is proposed. The type strain is AM23(T) (=KACC 18260(T) = DSM 29493(T)).

• 出版日期2016-4