Application of some room temperature ionic liquids in the development of Biosensors at carbon film electrodes

作者：Pauliukaite Rasa; Doherty Andrew P; Murnaghan Kevin D; Brett Christopher M A^*

来源：Electroanalysis, 2008, 20(5): 485-490.

DOI：10.1002/elan.200704081

• room temperature ionic liquids
• electrochemical enzyme biosensors
• sol-gel
• glutaraldehyde cross-linking
• poly(neutral red)
• glucose

Two room temperature ionic liquids, 1-butyl-3-methylimidazolium bistriflimide and 1-butyl-3-methylimidazolium nitrate, were employed for enzyme immobilization in a new sol-gel matrix and, for the first time, were successfully applied as electrolyte carriers in a biosensing system. The new sol-gel matrix, based on 3-aminopropyltrimethoxysilane and 1-butyl-3-methylimidazolium bistriflimide mixtures, did not crack even after several weeks when kept dry, and exhibited similar analytical properties to aqueous sol-gel based glucose biosensors. The linear range was up to 1.1 mM of glucose, sensitivity was 62 nA mM(-1) and the limit of detection was 28.8 mu M The optimum ionic liquid electrolyte carrier was found to be 1-butyl-3-methylimidazolium nitrate, where the biosensor was made by electrodeposition of the redox mediator, poly(neutral red), and the enzyme was immobilized by cross-linking with glutaraldehyde. The results showed that application of room temperature ionic liquids to biosensors is very promising and can be further exploited.

• 出版日期2008-3